DALTONbio has developed its Third Generation of Hybrid Capture technology with exclusive intellectual property rights. The technology allows to detect targets in either full-length genome or a specific fragment of nucleic acid by using their corresponding probes. The technology also allows genotyping of the targets when needed. The process is fully automated, eliminating the need for nucleic acid extractions and without gene amplifications, and can be easily performed in general laboratories.

Principle:

The target DNA is first denatured into single strands, the single stranded DNA binds to a specific RNA probe to form a DNA-RNA hybrid, the DNA-RNA hybrid is then captured by a specific anti-DNA-RNA antibody that has been immobilized on a capture microplate. The captured DNA-RNA hybrid will be recognized by a second anti-DNA-RNA antibody that has been linked with an enzyme. Enzymatic chemiluminescence amplifies the signal to achieve quantitative detection of the target load.

Technical Advantages:

Convenient - no nucleic acid extraction, no gene amplification, nucleic acid detection performed in general labs.

Accurate - genome-wide RNA probe designs, high sensitivity and specificity.

Automation - fully automated Hybridization Capture (DH-FA) system, fully integrated, "samples in, results out".